Tuberculosis preventive therapy (TPT) effectively decreases rates of developing active tuberculosis disease in people living with HIV (PLHIV) who are at increased risk. The Uganda Ministry of Health launched a 100-day campaign to scale-up TPT in PLHIV in July 2019. We sought to examine the effect of the campaign on trends of TPT uptake and char- acteristics associated with TPT uptake and completion among persons in HIV care. We ret- rospectively reviewed routinely collected data from 2016 to 2019 at six urban public health facilities in Uganda.